《植物生理学报》 2016, 52(7): 1011-1018

铝胁迫下质膜H⁺-ATPase对水稻硝态氮吸收的影响

周小华^1,2, 徐慧妮¹, 谷照虎¹, 陈丽梅¹, 李昆志^1,*

¹昆明理工大学生命科学与技术学院生物工程技术研究中心, 昆明650500; ²云南商务信息工程学校, 昆明650204

通信作者：李昆志；E-mail: likunzhi63@126.com

摘　要：

为了研究铝胁迫下水稻质膜H⁺-ATPase对硝态氮吸收影响, 以‘滇优35号’ (粳稻)为试验材料, 采用溶液培养法研究50 μmol•L^-1 Al³⁺胁迫下添加质膜H⁺-ATPase的激活剂壳梭孢素(FC)和抑制剂腺苷-5’-单磷酸(AMP)对质膜H⁺-ATPase水解活性和H⁺-泵活性, 质膜H⁺-ATPase和14-3-3蛋白的相互作用对水稻NO₃^--N吸收的影响。结果表明, AMP处理使铝胁迫下水稻质膜H⁺-ATPase水解活性、H⁺-泵的活性, 质膜H⁺-ATPase和14-3-3蛋白的相互作用下降, H₂O₂和MDA含量增加, 从而导致水稻NO₃^--N吸收降低。FC处理可使铝胁迫下水稻质膜H⁺-ATPase水解活性、H⁺-泵的活性以及质膜H⁺-ATPase和14-3-3蛋白的相互作用增加, H₂O₂和MDA含量下降, 从而使水稻NO₃^--N吸收增加。这些研究结果表明铝胁迫通过影响质膜H⁺-ATPase与14-3-3蛋白互作来影响质膜H⁺-ATPase水解活性和氢泵活性以及H₂O₂和MDA含量, 进而影响对硝态氮的吸收。

关键词：铝胁迫; 水稻; 质膜H⁺-ATP酶; 硝态氮; FC; AMP

收稿：2016-01-29   修定：2016-05-18

资助：国家自然科学基金(31260297和31560351)和云南省九湖水专项治理项目(KKK0201126044)。 * 通讯作者(E-mail: likunzhi63@126.com)。

Effects of PM H⁺-ATPase on the nitrate uptake of rice under aluminum stress

ZHOU Xiao-Hua^1,2, XU Hui-Ni¹, GU Zhao-Hu¹, CHEN Li-Mei¹, LI Kun-Zhi^1,*

¹Biotechnology Research Centre, Faculty of Life Science and Biotechnology, Kunming University of Science and Technology, Kunming 650500, China; ²Yunnan Business Information Engineering School, Kunming 650204, China

Corresponding author: LI Kun-Zhi; E-mail: likunzhi63@126.com

Abstract:

The aim of the experiment was to study the effects of the plasma membrane (PM) H⁺-ATPase on the nitrate (NO₃^--N) uptake of rice under aluminum (Al) stress. A hydroponic experiment was conducted with the rice hybrid ‘Dian superior 35’ (Hybrid, Japonica) to study the effects of activator Fusicoccin (FC) and inhibitor Adeosine- 5’-Monophosphate (AMP) of PM H⁺-ATPase on the activities of PM H⁺-ATPase hydrolysis and H⁺- pump and the interaction of PM H⁺- ATPase and 14-3-3 protein on the uptake of NO₃^--N in root of the rice under 50 μmol•L^-1 Al stress. The results showed that Al combing with AMP treatment could decrease the activities of PM H⁺-ATPase hydrolytic and H⁺-pump and the interaction of PM H⁺-ATPase and 14-3-3 protein and enhanced the contents of H₂O₂ and malondialdehyde (MDA), which decreased the NO₃^--N uptake; Al combining with FC treatment could increase the activities of both PM H⁺-ATPase hydrolysis and H⁺-pump, decreased the contents of H₂O₂ and MDA, which promoted the NO₃^--N uptake of rice compared with single Al treatment. These results indicated that Al stress affects the activities of PM H⁺-ATPase hydrolytic and H⁺-pump as well as the contents of H₂O₂ and MDA by influencing the interaction of PM H⁺-ATPase and 14-3-3 protein, thereby affecting the absorption of nitrate.

Key words: aluminum (Al) stress; rice; plasma membrane H⁺-ATPase; NO₃^--N; FC; AMP